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目的制备抗EOLA1多克隆抗体并检测其在肿瘤组织中的表达。方法采用原核表达获得EOLA1蛋白,纯化后作为抗原免疫大白兔,获得兔抗人EOLA1(hEOLA1)多抗血清,进行免疫组化检测人多肿瘤芯片上EOLA1的表达。结果制备了高滴度的hEOLA1多克隆抗体,免疫组化显示EOLA1蛋白在肺癌、结肠癌、胃癌和肝癌仅表达于肿瘤间质,肿瘤细胞本身未见表达,而在胶质瘤和乳腺癌则呈广泛表达。结论EOLA1可能在胶质瘤和乳腺癌的发生、发展方面起着重要作用。
Objective To prepare anti-EOLA1 polyclonal antibody and test its expression in tumor tissue. Methods EOLA1 protein was obtained by prokaryotic expression. After being purified, it was used as antigen to immunize rabbits. Rabbit anti-human EOLA1 antiserum was obtained and immunohistochemistry was used to detect the expression of EOLA1 on human multi-tumor oncology chip. Results High titer hEOLA1 polyclonal antibody was prepared. Immunohistochemistry showed that EOLA1 protein was only expressed in tumor stroma, lung cancer, colon cancer, gastric cancer and liver cancer, but not in tumor cells itself, while in glioma and breast cancer Was widely expressed. Conclusion EOLA1 may play an important role in the occurrence and development of glioma and breast cancer.