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番茄黄化曲叶病(tomato yellow leaf curl disease, TYLCD)是番茄上的重要病害, 可由多种双生病毒引起。为了明确北京地区番茄黄化曲叶病的病毒种类和病毒基因组变异情况, 本研究收集了51个采自北京不同区县的病毒疑似样品, 进行了双生病毒的检测及分离物的基因组分析。经双生病毒简并引物检测25个样品呈阳性; 扩增获得10个代表性分离物的全基因组, 大小均为2 781 bp, 共含有6个开放阅读框; 经比对, 10个分离物与Tomato yellow leaf curl virus-Israel (TYLCV-IL)同源率大于99%; 基于我国TYLCV分离物全基因组序列的变异分析表明, TYLCV在进化上比较保守, 整个基因组上的变异是不均匀的; 系统发育树显示我国TYLCV分离物在进化树上可分为3个组群, 分析了我国TYLCV群体进化情况。
Tomato yellow leaf curl disease (TYLCD) is an important disease in tomato and can be caused by a variety of geminiviruses. In order to clarify the virus types and virus genome variation of tomato yellow leaf curl in Beijing, 51 suspected virus samples collected from different districts and counties of Beijing were collected for the detection of geminiviruses and the genomic analysis of isolates. Twenty-five samples were positive by twin degenerate primers. The whole genome of 10 representative isolates was amplified with a total length of 2 781 bp and contained 6 open reading frames. By comparison, 10 isolates The homology analysis showed that TYLCV-IL was more than 99% homologous to Tomato yellow leaf curl virus-Israel (TYLCV-IL). Based on the genome-wide variation analysis of TYLCV isolates in China, TYLCV was conserved in evolution and the whole genome was heterogeneous. The phylogenetic tree shows that TYLCV isolates in China can be divided into three groups on the phylogenetic tree, and the evolution of TYLCV population in China is analyzed.