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目的对某集团公司食堂发生的一起食物中毒病原菌进行实验室检测。方法参照GB/T4789-2008、WS/T8-1996、GB17012-1992和《卫生防疫检验》对采集的47份样本进行肠道致病菌检测,对检出的副溶血性弧菌用VITEK全自动微生物分析系统进行生化鉴定,以及神奈川试验、血清分群分型及药敏试验。结果从47份样本中检出40株副溶血性弧菌,检出率高达85.1%,神奈川试验均阳性,生化反应呈五种模式,血清分群有O3、O4、O1三种,血清分型有K6、K8、K29、K68、KUT五种,均对氨苄西林、头孢噻吩、阿莫西林/克拉维酸耐药,对头孢曲松、头孢哌酮、头孢克肟、阿米卡星、氟派酸、复方新诺明、链霉素、庆大霉素、环丙沙星、亚胺培南、氯霉素等药物敏感。结论本次食物中毒是由副溶血性弧菌引起。
Objective To conduct laboratory tests on food poisoning pathogens that occurred in the canteen of a group company. Methods According to GB / T4789-2008, WS / T8-1996, GB17012-1992 and “Health and Epidemic Prevention Test”, 47 samples collected were tested for intestinal pathogenic bacteria. Vibrio parahaemolyticus was detected by VITEK automatic Microbial analysis system for biochemical identification, as well as Kanagawa test, serological classification and susceptibility testing. Results 40 strains of Vibrio parahaemolyticus were detected in 47 samples, the detection rate was as high as 85.1%. The results of Kanagawa test were positive. The biochemical reactions showed five patterns. The serum samples were divided into three groups: O3, O4 and O1. K6, K8, K29, K68, KUT five kinds of resistance to ampicillin, cefalotin, amoxicillin / clavulanic acid resistance to ceftriaxone, cefoperazone, cefixime, amikacin, fluorine school Acid, cotrimoxazole, streptomycin, gentamicin, ciprofloxacin, imipenem, chloramphenicol and other drugs sensitive. Conclusion This food poisoning is caused by Vibrio parahaemolyticus.