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目的:探讨电磁脉冲(electromagnetic pulses,EMP)对孕期小鼠及其胚胎发育的影响。方法:采用不同场强的EMP(分别为0、50、100、200、400 kV·m-1)辐照器官形成期的BALB/c孕鼠,于孕18天解剖小鼠,测量孕鼠体重增长值、脏器/体重,胎盘重、胎鼠体重、身长、尾长,并记录吸收胎、死胎、生长发育迟缓及畸胎的数量。结果:各辐照剂量组孕鼠体重增长值、脏器/体重与对照组相比均无显著性差异(P>0.05);胎盘重、胎鼠体重、身长、尾长数值明显低于对照组(P<0.01)。50、400 kV·m-1和100、200、400 kV·m-1辐照组可分别导致死胎率和生长发育迟缓率增加(P<0.05),在400 kV·m-1的EMP辐照组中,畸胎数也有升高的趋势,其中,畸胎主要表现为肢体和骨发育异常。结论:本实验条件下,不同场强的EMP辐照可对器官形成期小鼠胚胎的生长发育产生一定的影响,胚胎肢芽及骨发育可能是EMP作用的特殊靶点。
Objective: To investigate the effects of electromagnetic pulses (EMP) on the development of embryos and embryos of pregnant mice. Methods: BALB / c pregnant mice irradiated with EMP (0, 50, 100, 200 and 400 kV · m-1, respectively) at different field intensities were dissected for 18 days and their body weight Growth value, organ / body weight, placental weight, fetal rat weight, length, tail length, and record the number of absorbed fetus, stillbirth, growth retardation and teratogenicity. Results: There was no significant difference in body weight gain and organ / body weight between the pregnant rats and the control group (P> 0.05). The weight of the placenta and fetal rat body weight, body length and tail length were significantly lower than those of the control group (P <0.01). At 50, 400 kV · m-1 and 100, 200 and 400 kV · m-1 irradiation groups, the stillbirth rate and growth retardation rate increased respectively (P <0.05) In the group, the number of teratostails also tended to increase. Among them, the teratosperms mainly manifested as limbs and bone dysplasia. CONCLUSION: Under the experimental conditions, EMP irradiation with different field intensities may have some effects on the growth and development of mouse embryos during organogenesis. The embryonic limb buds and bone development may be the special target of EMP.