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目的探讨N-甲基-N’-硝基-N-亚硝基胍(MNNG)对人支气管上皮16HBE细胞周期进程的影响及相关分子机制。方法 16HBE细胞经MNNG处理后,噻唑蓝(MTT)法检测MNNG对16HBE细胞增殖的影响,PI染色检测细胞凋亡,Hoechst 33342和PI双染检测细胞坏死,流式细胞术检测细胞周期分布,在周期变化明显的时点应用蛋白免疫印记检测周期相关蛋白含量变化。结果 MNNG能够剂量依赖性的抑制细胞增殖和诱导凋亡,亚致死剂量的MNNG使细胞引起明显的S期和G2/M期阻滞,同时细胞周期相关蛋白p-Cdk2(Thr160)和Cdc25A含量下降明显。结论 MNNG通过降低细胞p-Cdk2(Thr160)和Cdc25A的含量诱导16HBE细胞发生S期和G2/M期阻滞。
Objective To investigate the effects of N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) on the cell cycle progression of human bronchial epithelial cells 16HBE and related molecular mechanisms. Methods 16HBE cells were treated with MNNG. MTT assay was used to detect the effect of MNNG on the proliferation of 16HBE cells. The apoptosis of 16HBE cells was detected by PI staining. Cell necrosis was detected by Hoechst 33342 and PI double staining. Cell cycle distribution was analyzed by flow cytometry. Changes in the cycle of significant changes in the application of protein immunoblotting cycle-related protein content changes. Results MNNG inhibited cell proliferation and induced apoptosis in a dose-dependent manner. Sub-lethal doses of MNNG caused significant S phase arrest and G2 / M arrest in cells, and decreased the expression of p-Cdk2 (Thr160) and Cdc25A obvious. Conclusion MNNG induces S phase and G2 / M arrest in 16HBE cells by decreasing the content of p-Cdk2 (Thr160) and Cdc25A.