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在单倍体育种的研究上寻找合适的培养基成分是十分重要的,因为一定成分的培养基是促使花粉发育成单倍体植株的最重要的外界因素。我们最初开展烟草花药培养采用的是国外的尼许(Nitsch 1969)培养基,并附加一定浓度的吲哚乙酸。但是尼许培养基由20余种化学药品所组成,成分比较复杂,配制手续繁琐,而且有些药品价格昂贵,又不易买到,大大限制了这一技术的应用和推广。我们遵照毛主席关于“独立自主,自
It is important to find a suitable culture medium in the study of haploid breeding because a medium of certain composition is the most important external factor that promotes pollen to develop into haploid plants. We initially conducted tobacco anther culture using a foreign Nitsch 1969 medium with a certain concentration of indole acetic acid. However, Ni Xu culture medium consists of more than 20 kinds of chemical composition, the composition is more complicated preparation procedures cumbersome, and some drugs are expensive, but not easy to buy, greatly limiting the application and promotion of this technology. We follow Chairman Mao’s principle of “being independent and self-reliant.”