The global“myopia boom”has raised significant international concerns.Despite a higher myopia preva-lence in Asia,previous large-scale genome-wide association studies(GWASs)were mostly based on Eu-ropean descendants.Here,we report a GWAS of spherical equivalent(SE)in 1852 Chinese Han individuals with extreme SE from Guangzhou(631＜-6.00D and 574＞0.00D)and Wenzhou(593＜-6.00D and 54＞-1.75D),followed by a replication study in two independent cohorts with totaling 3538 East Asian individuals.The discovery GWAS and meta-analysis identify three novel loci,which show genome-wide significant associations with SE,including 1q25.2 FAM163A,10p11.22 NRP1/PRAD3,and 10p11.21 ANKRD30A/MTRNR2L7,together explaining 3.34％of SE variance.10p11.21 is successfully replicated.The allele frequencies of all three loci show significant differences between major continental groups(P＜0.001).The SE reducing(more myopic)allele of rs10913877(1q25.2 FAM163A)demonstrates the highest frequency in East Asians and much lower frequencies in Europeans and Africans(EAS=0.60,EUR=0.20,and AFR=0.18).The gene-based analysis additionally identifies three novel genes associated with SE,including EI24,LHX5,and ARPP19.These results provide new insights into myopia pathogenesis and indicate the role of genetic heterogeneity in myopia epidemiology among different ethnicities.