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目的探讨PTGER4基因单核苷酸多态性(SNP)与山东汉族人群强直性脊柱炎(AS)的关系。方法利用Taq Man-PCR基因分型法对600例AS患者(AS组)和620例正常健康人(正常对照组)PTGER4基因SNPs位点rs7726237、rs4133101和rs16870224进行基因分型,计算各位点基因型和等位基因频率,比较两组各位点之间基因型和等位基因频率分布差异。结果 AS组与正常对照组PTGER4基因rs7726237位点A等位基因分布频率(78.5%,74.7%)和G等位基因分布频率(21.5%,25.3%)的分布不同,差异有统计学意义(P=0.026,OR=0.81,95%CI:0.67~0.97);AS组GG、AG、AA基因型频率分布(5.3%、32.3%、62.3%)与对照组(5.2%、40.3%、54.5%)相比差异有统计学意义(P=0.014,OR=0.90,95%CI:0.54~1.51;P=0.014,OR=0.70,95%CI:0.55~0.89)。PTGER4基因rs4133101位点和rs16870224位点SNP在AS组和正常对照组差异均无统计学意义(P>0.05)。单倍体型分析与AS的易感性无关(P>0.05)。结论 PTGER4基因与山东汉族人群AS的易感性相关。
Objective To investigate the relationship between PTGER4 single nucleotide polymorphism (SNP) and ankylosing spondylitis (AS) in Shandong Han population. Methods Genotypes of SNPs rs7726237, rs4133101 and rs16870224 of PTGER4 gene in 600 patients with AS (AS group) and 620 healthy controls (normal control group) were genotyped by Taq Man-PCR genotyping. The genotypes of each locus And allele frequency, genotype and allele frequency distribution differences between the two groups were compared. Results The frequency of allele A (78.5%, 74.7%) and allele G (21.5%, 25.3%) of PTGER4 rs7726237 in AS group and normal control group were significantly different (P (5.3%, 32.3%, 62.3%) and control group (5.2%, 40.3%, 54.5%) in the AS group were significantly higher than those in the control group (P = 0.026, OR = 0.81, 95% CI: 0.67-0.97) The difference was statistically significant (P = 0.014, OR = 0.90, 95% CI: 0.54-1.51; P = 0.014, OR = 0.70, 95% CI: 0.55-0.89). The rs4133101 and rs16870224 SNPs of PTGER4 gene were not significantly different between AS group and normal control group (P> 0.05). Haplotype analysis was not related to susceptibility to AS (P> 0.05). Conclusion The PTGER4 gene is associated with the susceptibility to AS in Shandong Han population.