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本文采用离体兔门静脉环的方法,观察了普萘洛尔(Propranolol,Pro)和维拉帕米(Verapamil,Ver)对门静脉血管的作用。结果表明,Pro对兔门静脉环正常张力无影响。Pro和Ver使兔门静脉环KCl和C_αC_2量效曲线非平行右移,最大反应压低,显示非竞争性拮抗的两种曲线。显著抑制高K~+去极化所致门静脉环的收缩作用。此外Pro100μmol/L和Ver10μmol/L显著抑制兔门静脉条的自律性收缩,而实验证明这种收缩是钙依赖性的。提示较大剂量的Pro可能通过阻断细胞膜上电压依赖通道而发挥钙拮抗作用。
In this study, we observed the effects of propranolol (Pro) and verapamil (Ver) on portal vein in vitro. The results showed that Pro had no effect on the normal tension of rabbit’s portal vein. Pro and Ver had a non-parallel shift of the dose-response curve of KCl and C_αC_2 in the portal vein of rabbits to the right and the maximum response pressure was low, showing two curves of noncompetitive antagonism. Significant inhibition of high K ~ + depolarization caused by the contraction of the portal vein. In addition Pro100μmol / L and Ver10μmol / L significantly inhibit the voluntary contractions of rabbit portal vein strips, and experiments show that this contraction is calcium-dependent. Suggesting that higher doses of Pro may exert calcium antagonism by blocking voltage-dependent channels on the cell membrane.