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目的 诱导慢性移植物抗宿主病狼疮样小鼠模型。方法 选用 (DBA 2×C57BL 6J)F1小鼠 ,通过尾静脉注射其母鼠DBA 2淋巴细胞诱导模型 ,观察 12周。结果 F1小鼠注射母鼠淋巴细胞后 2周产生自身抗体 ,出现蛋白尿 ,4周血脂、血肌酐、尿素氮轻度升高 ,肾脏病理仅有系膜细胞轻度增生 ,未见间质损害。 8周血生化指标明显改变 ,肾脏病理示肾小球系膜细胞中度增生 ,间质炎细胞浸润和肾小管大量蛋白管型 ,10～ 12周各项观察指标改变明显 ,病理出现局灶或弥漫肾小球硬化 ,免疫荧光示IgG、IgM、C3沿毛细血管壁及系膜区沉积。结论 慢性移植物抗宿主病狼疮样小鼠模型类似人类狼疮性肾炎 ,是良好的狼疮肾炎模型
Objective To induce a chronic graft-versus-host disease-like lupus-like mouse model. Methods (DBA 2 × C57BL 6J) F1 mice were used to induce DBA 2 lymphocytes in their maternal mice via tail vein injection for 12 weeks. Results The autoantibodies and proteinuria occurred in the F1 mice 2 weeks after the injection of maternal lymphocytes. Proteinuria, serum lipids, serum creatinine and urea nitrogen were slightly elevated in 4 weeks. Mild renal hyperplasia was observed in renal pathology with no interstitial damage . 8 weeks of biochemical indicators of blood was significantly changed, renal pathology showed mesangial cells moderate proliferation, interstitial inflammatory cell infiltration and large tubular protein tubular, 10 to 12 weeks of various indicators of change significantly, the pathological appearance of focal or Diffuse glomerulosclerosis, immunofluorescence showed IgG, IgM, C3 deposition along the capillary wall and mesangial area. Conclusion The chronic graft-versus-host disease lupus-like mouse model resembles human lupus nephritis and is a good lupus nephritis model