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目的:探讨高渗盐水复苏对失血性休克家兔胃粘膜及肝细胞能量代谢影响的机制.方法:家兔按20ml/kg体重放血致失血性休克,60min后分别回输10%放血量的抗凝自体全血、生理盐水及1282mmol/L氯化钠高渗盐水.采用高效液相色谱技术检测胃底粘膜细胞及肝细胞ATP,ADP,AMP含量.结果:高渗盐水复苏后,失血性休克家兔胃底粘膜细胞及肝细胞内ATP含量[分别为(217.63±70.29)nmol/g和(178.72±53.65)nmol/g]非常显著地高于缺血对照组[(87.88±43.61)nmol/g和(78.14±24.25)nmol/g](P<0.01),能荷(分别为0.286±0.051和0.213±0.002)显著高于缺血对照组动物(0.175±0.095和0.126±0.036)(P<0.05).结论:高渗盐水复苏,具有改善失血性休克家兔胃底粘膜细胞及肝细胞能量代谢的作用
Objective: To investigate the mechanism of hypertonic saline resuscitation on the energy metabolism of gastric mucosa and hepatocytes in hemorrhagic shock rabbits. Methods: Rabbits were transfused with 20ml / kg body weight to induce hemorrhagic shock. After 60 minutes, 10% blood volume anticoagulant whole blood, normal saline and 1282mmol / L sodium chloride hypertonic saline were respectively administered. High-performance liquid chromatography (HPLC) was used to detect ATP, ADP and AMP in gastric mucosal cells and hepatocytes. Results: After resuscitation with hypertonic saline, the content of ATP in the gastric mucosal cells and hepatocytes of hemorrhagic shock rabbits [(217.63 ± 70.29) nmol / g and (178.72 ± 53.65) nmol / g] were significantly higher than those in ischemic control group [(87.88 ± 43.61) nmol / g and (78.14 ± 24.25) nmol / g], respectively 0.286 ± 0.051 and 0.213 ± 0.002) were significantly higher than those in ischemic control animals (0.175 ± 0.095 and 0.126 ± 0.036, respectively) (P <0.05). Conclusion: The resuscitation of hypertonic saline can improve the gastric mucosal cells and the energy metabolism of hepatocytes in hemorrhagic shock in rabbits