目的　为确定我国南澎列岛是否为恙虫病疫源地 ,对来自该地的 8个恙虫病东方体分离株进行基因鉴定。方法　应用套式聚合酶链反应 (NPCR)和限制片段长度多态性分析 (RFLP) ,NPCR的引物来自恙虫病东方体5 6kDa蛋白基因 ,NPCR阳性产物由HincII和PstI消化并产生特异性酶切图谱并予以分析。结果　南澎列岛的 8个恙虫病东方体分离株属三个型 ,即Karp ,Kato和一新型恙虫病东方体。结论　南澎列岛是恙虫病疫源地 Objective To identify whether Nanchong Islands in China is a source of scrub typhus or not and to identify eight isolates of Orientia tsutsugamushi from this region. Methods The NPCR and restriction fragment length polymorphism (RFLP) analysis were used. The primers of NPCR were from the 56 kDa protein gene of Orientia tsutsugamushi, and the positive product of NPCR was digested with HincII and PstI to generate specific digestion Map and analyze. Results Eight oriental isolates of tsutsugamushi were found in Nanpeng Islands, belonging to three types, Karp, Kato and a new type of oriental tsutsugamushi. Conclusion Nanpeng Islands is the source of scrub typhus