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目的研究决明子提取物对高血脂模型小鼠血脂水平和聚脂基因转录表达的影响。方法用两种不同剂量的决明子提取物对小鼠进行sc给药,分别于1、2、4 h摘眼球取血后处死。试剂盒检测血脂生化指标,荧光定量PCR技术检测聚脂相关基因的转录表达规律。结果决明子提取物处理的高血脂模型小鼠,总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)均有显著(P<0.05)或非常显著(P<0.01)降低,高密度脂蛋白胆固醇(HDL-C)非常显著升高(P<0.01),总体上低剂量处理效果较好。脂肪组织中决明子提取物上调过氧化物酶体增殖物激活受体γ(PPARγ)、固醇调节元件结合蛋白(SREBP-1c)、激素敏感性脂酶(HSL)和甘油三酯水解酶(TGH)的转录表达,1 h达非常显著(P<0.01),下调脂肪酸合成酶(FAS),同样在1 h达非常显著(P<0.01)。肌肉中决明子提取物下调PPARγ、SREBP-1c、HSL和TGH的转录表达,其中PPARγ在2 h达非常显著(P<0.01),SREBP-1c、HSL在1 h达非常显著(P<0.01),TGH在2 h达非常显著(P<0.01),上调FAS转录表达且1 h达非常显著(P<0.01)。结论决明子提取物可调节高血脂模型小鼠的血脂,并通过调控脂肪组织和肌肉中脂代谢相关基因的转录表达来调节动物体脂代谢。
Objective To study the effect of extract of Cassia seed on the level of lipids and the transcriptional expression of lipids in hyperlipidemia mice. Methods The mice were sc administered with two different doses of extract of Cassia seed, and the blood was taken off at 1, 2 and 4 h after sacrifice. The kit was used to detect the biochemical indexes of blood lipids, and the fluorescence quantitative PCR was used to detect the transcriptional expression of the related genes. Results The levels of total cholesterol (TC), triglyceride (TG) and low density lipoprotein cholesterol (LDL-C) were significantly (P <0.05) or very significant ), High-density lipoprotein cholesterol (HDL-C) was significantly increased (P <0.01), the overall effect of low-dose treatment is better. Cassia seed extract in adipose tissue up-regulates peroxisome proliferator-activated receptor γ (PPARγ), sterol regulatory element binding protein (SREBP-1c), hormone sensitive lipase (HSL) and triglyceride hydrolase (P <0.01), down-regulated fatty acid synthase (FAS) at 1 h (P <0.01). Camptothecin extract in muscle reduced transcriptional expression of PPARγ, SREBP-1c, HSL and TGH, PPARγ was significantly up-regulated at 2 h (P <0.01), SREBP-1c and HSL at 1 h were significantly increased TGH was very significant at 2 h (P <0.01), and up-regulated the expression of FAS at 1 h (P <0.01). Conclusion Cassia seed extract can regulate blood lipids in hyperlipidemic mice and regulate lipid metabolism in animals by regulating the transcriptional expression of lipid metabolism related genes in adipose tissue and muscle.