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小鼠是常用的实验动物,尤其广泛应用于药理研究及致病因子对机体影响方面,皮肤学专业也不例外.小鼠尾巴皮肤长有细而短的毛发,但机体其它部位长满粗而长的毛发,因此小鼠尾巴皮肤就成为药物皮肤反应、皮肤病理及药物对皮肤病疗效的很好观察部位.小鼠尾巴皮肤很薄且较长,并且一个自然的生理性弧度,若制作与表皮垂直的横切片容易的话,制作与尾巴长轴平行的纵切片则很困难,我们曾作过多次,最后均以失败而告终.但从制作纵切片比横切片可扩大观察面积的角度出发,我们反复摸索成功地制成了小鼠尾巴皮肤的纵切片100例,都获得了满意效果.1 材料与方法 取小鼠尾巴皮肤,常规固定修材.然后取一载玻片,两面平整铺上4～5层棉纱,将标本凹面朝向棉纱,一一平铺于棉纱之上,用细棉线稍加固定以防标本脱离棉纱.把固扎有标本的载玻片横立于卧式染色缸之中,进行逐级脱水直至脱水过程结束.然后再把标本从载玻片上取下来,进行透明、包埋,行垂直于表皮的纵切片、脱蜡、染色、封片.2 结果 经此方法制作的不鼠尾巴皮肤纵切片面积较横切片扩大了数倍,表皮、真皮结构完好,表层、真皮层次清楚呈典型垂直表皮切面状.3 讨论 皮肤由上皮组织组成表层的皮肤与结缔组织构成的深层真层组成.此二种组织对脱水剂的缩水度不同极易造成表皮向
Mice are commonly used laboratory animals, in particular, widely used in pharmacological research and pathogenic factors on the impact of the body, dermatology is no exception.Mouse tail skin has thin and short hair, but the rest of the body covered with coarse Long hair, so the mouse tail skin has become a drug skin reactions, skin pathology and drugs on skin diseases a good place to observe the effect of the mouse tail skin is thin and long, and a natural physiological curvature, if the production and It is very difficult to make longitudinal slices parallel to the long axis of the tail, because we have done it many times before finally failing. However, from the perspective of making the longitudinal section wider than the transverse section, , We repeatedly explored successfully made mouse tail skin longitudinal section of 100 cases, have obtained satisfactory results.1 Materials and methods to take the tail skin of mice, routine fixed repair.And then take a slide, flat on both sides of the shop 4 to 5 layers of cotton yarn, the specimen concave toward the cotton yarn, one by one tile on the cotton yarn, slightly fine cotton thread fixed to prevent the specimen from the cotton yarn. It , Dewatering step by step until the end of the dehydration process and then the specimen was removed from the slide, transparent, embedded, vertical line of the skin longitudinal slitting, dewaxing, dyeing, sealing sheet .2 results prepared by this method The area of longitudinal section of rat’s tail skin is several times larger than that of transverse section, the structure of epidermis and dermis is intact, and the surface and dermis level is clear and typical of vertical epidermis.3 Discussion The deep layer of skin and connective tissue composed of epithelial tissue Layer composed of these two organizations on the dehydration agent shrinkage can easily lead to the epidermis