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目的:研究PPAR-γ基因表达对前列腺癌细胞增殖和肿瘤特殊糖酵解代谢的影响。方法:采用RNAi技术构建PPARγ低表达的腺病毒载体并转染到前列腺癌细胞株,并以空白载体为对照组,进行细胞增殖检测、细胞凋亡检测、糖酵解代谢基因及产物乳酸检测,并比较两组之间的结果差异。结果:经RNAi抑制的PPAR-γ前列腺癌细胞株与对照组相比,蛋白表达量降低至(26.00±4.06)%,增殖抑制率最大为第2天达(39.50±4.92)%,细胞凋亡率升高至(21.03±3.08)%,糖酵解代谢基因产物(Myc和Glut-1)下降,第4天培养液中乳酸浓度为对照组的69.71%,上述结果具有统计学差异(P<0.01)。结论:抑制PPAR-γ基因的表达有望成为治疗前列腺癌新方法。
Objective: To investigate the effect of PPAR-γ gene expression on the proliferation of prostate cancer cells and the tumor specific glycolytic metabolism. Methods: The adenovirus vector with low expression of PPARγ was constructed and transfected into prostate cancer cell lines by RNAi technique. Cell proliferation assay, apoptosis assay, glycolysis gene and lactic acid assay were performed with blank vector as control. And compare the difference between the two groups. Results: Compared with the control group, the expression of PPAR-γprotein in RNAi group was reduced to (26.00 ± 4.06)% and the highest proliferation inhibition rate was (39.50 ± 4.92)% on the second day. (21.03 ± 3.08)%, the products of glycolytic metabolism (Myc and Glut-1) decreased, and the concentration of lactic acid in the culture medium on day 4 was 69.71% of that in control group. The above results were statistically significant (P < 0.01). Conclusion: Suppression of PPAR-γ gene expression is expected to become a new method for the treatment of prostate cancer.