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目的:研究多房棘球绦虫重组Bb-EmⅡ/3-Em14-3-3疫苗对原头蚴攻击小鼠细胞因子变化的影响。方法:将重组Bb-EmⅡ/3-Em14-3-3疫苗分别通过皮下注射、肌肉注射、鼻腔黏膜接种和口服接种免疫BALB/c小鼠12周后,用50个多房棘球绦虫原头蚴腹腔注射进行攻击,攻击感染后18周剖杀小鼠,取脾脏制备淋巴细胞悬液体外培养,分别以多房棘球绦虫抗原(EmAg)、刀豆素A(ConA)刺激诱生白细胞介素12(IL-12)、白细胞介素10(IL-10)和干扰素γ(IFN-γ);以脂多糖(LPS)刺激诱生肿瘤坏死因子α(TNF-α)。常规ELISA测定脾细胞培养上清液中IL-12、IL-10、IFN-γ和TNF-α水平。结果:与对照组相比,疫苗免疫组IFN-γ、IL-12、TNF-α水平增加,IL-10水平降低,EmAg刺激组和ConA或LPS刺激组细胞因子水平明显高于相应的原液组。结论:多房棘球绦虫重组Bb-EmⅡ/3-Em14-3-3疫苗可诱导攻击感染小鼠产生Th1型细胞免疫应答,增强宿主抗多房棘球绦虫感染的保护性免疫反应。
Objective: To study the effects of recombinant Bb-EmⅡ / 3-Em14-3-3 vaccine on the changes of cytokines in mice challenged with Echinococcus multilocularis. Methods: BALB / c mice were immunized with recombinant Bb-EmⅡ / 3-Em14-3-3 vaccine by subcutaneous injection, intramuscular injection, nasal mucosal inoculation and oral inoculation respectively for 12 weeks. Fifty Echinococcus multilocularis The mice were challenged by intraperitoneal injection of the larvae. The mice were killed 18 weeks after the challenge and the spleen lymphocytes were prepared and cultured in vitro. Erythrocytes were induced by EmAg and ConA stimulation respectively. (IL-12), interleukin 10 (IL-10) and interferon gamma (IFN-γ) were stimulated with lipopolysaccharide (LPS) to induce tumor necrosis factor alpha (TNF- alpha). The levels of IL-12, IL-10, IFN-γ and TNF-α in spleen cell culture supernatant were determined by routine ELISA. Results: Compared with the control group, the levels of IFN-γ, IL-12 and TNF-α increased and the levels of IL-10 decreased in the immunized group. The levels of cytokines in EmAg-stimulated group and ConA- . CONCLUSION: Recombinant Bb-EmⅡ / 3-Em14-3-3 vaccine of Echinococcus multilocularis can induce Th1-type cellular immune response in challenge-infected mice and enhance host immune response against Echinococcus granulosus infection.