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目的探讨尿路上皮癌胚抗原1(UCA-1)在胰腺癌细胞侵袭转移中的作用。方法采用实时荧光定量PCR检测5株胰腺癌细胞长链非编码核糖核酸UCA-1的表达;采用UCA-1表达质粒提高PaTu8988细胞的UCA-1水平,小干扰RNA用于降低BxPC-3细胞的UCA-1表达。用Transwell侵袭实验和迁移实验观察转染后的PaTu8988细胞和BxPC-3细胞的侵袭和迁移能力变化,Western blot检测基质金属蛋白酶2(MMP-2)和MMP-9的表达。结果 UCA-1差异性表达于5株胰腺癌细胞。过表达UCA-1后,PaTu8988细胞的MMP-2和MMP-9的蛋白水平均增加,侵袭和迁移能力增强(P<0.001);而干扰UCA-1后,BxPC-3细胞的MMP-2和MMP-9的蛋白表达均降低,侵袭和迁移能力减弱(P<0.001)。结论 UCA-1的高表达能增强胰腺癌细胞的体外侵袭转移能力。
Objective To investigate the role of UCA-1 in invasion and metastasis of pancreatic cancer cells. Methods Real-time fluorescent quantitative PCR was used to detect the expression of UCA-1, a long-chain non-coding RNA in 5 pancreatic cancer cells. UCA-1 expression plasmid was used to increase UCA-1 level in PaTu8988 cells. Small interfering RNA UCA-1 expression. The invasion and migration of PaTu8988 cells and BxPC-3 cells were detected by Transwell invasion assay and migration assay. The expressions of MMP-2 and MMP-9 were detected by Western blot. Results UCA-1 was differentially expressed in 5 pancreatic cancer cells. After UCA-1 was overexpressed, the protein levels of MMP-2 and MMP-9 in PaTu8988 cells were increased, and the invasion and migration of PaTu8988 cells were enhanced (P <0.001) MMP-9 protein expression decreased, invasion and migration decreased (P <0.001). Conclusion The high expression of UCA-1 enhances the invasion and metastasis of pancreatic cancer cells in vitro.