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目的分析PTEN和LKB1在非小细胞肺癌中的表达特点以及二者的相关性。方法收集74例非小细胞肺癌患者蜡块,以免疫组织化学法检测PTEN及LKB1的表达;通过分析临床相关指标与PTEN及LKB1表达的相关性,探讨PTEN及LKB1表达的临床意义。结果 PTEN和LKB1的表达阳性率分别为47.3%和70.3%,LKB1表达阴性样本多伴有PTEN阴性表达,统计学分析发现LKB1与PTEN表达呈正相关(r=0.287,P=0.017);PTEN和LKB1在非小细胞肺癌中的表达与性别、年龄、病理类型无关(P均>0.05),PTEN表达缺失与淋巴结转移(P=0.017)、侵袭(P=0.008)及临床分期(P=0.019)相关,LKB1的表达缺失与肺癌侵袭(P=0.043)和临床分期(P=0.003)相关;联合PTEN和LKB1表达情况分析,同时为阳性表达的肺癌病例出现淋巴结转移和/或侵袭的比例低于同时为阴性表达者。结论 PTEN和LKB1的表达与非小细胞肺癌的发生及恶性进展有关,且可能存在协同作用;检测两个蛋白表达可作为非小细胞肺癌预后的判断依据。
Objective To analyze the expression of PTEN and LKB1 in non-small cell lung cancer and their correlation. Methods Totally 74 cases of non-small cell lung cancer patients were collected and the expression of PTEN and LKB1 were detected by immunohistochemical method. The clinical significance of PTEN and LKB1 expression was analyzed by analyzing the correlation between clinically relevant indexes and PTEN and LKB1 expression. Results The positive rates of PTEN and LKB1 were 47.3% and 70.3%, respectively. Most of the LKB1 negative samples were associated with PTEN negative expression. Statistical analysis showed that LKB1 and PTEN expression were positively correlated (r = 0.287, P = 0.017) The expression of PTEN was not associated with lymph node metastasis (P = 0.017), invasion (P = 0.008) and clinical stage (P = 0.019) in non-small cell lung cancer (NSCLC) . The loss of LKB1 expression was correlated with the invasion of lung cancer (P = 0.043) and clinical stage (P = 0.003). The expression of PTEN and LKB1 was correlated with the expression of LKB1 in both lung cancer patients with positive expression of lymph node metastasis and / or invasion less than Negative expression. Conclusions The expressions of PTEN and LKB1 are associated with the occurrence and malignant progression of non-small cell lung cancer and may have synergistic effects. To detect the expression of two proteins can be used as the basis for prognosis of non-small cell lung cancer.