Molecular cloning and expression analysis of FTZ-F1 in the GIFT tilapia,Oreochromis niloticus

来源 :动物学研究 | 被引量 : 0次 | 上传用户:hongyu203311
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The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination.We isolated the homologue of FTZ-F1 in genetically improved farmed tilapia(gfFTZ-F1).The full-length cDNA was isolated from the ovary,which included an open reading frame encoding a predicted protein of 486 amino acids.Sequence,tissue distribution and phylogenic analysis of the FTZ-F1 showed that the gfFTZ-F1 belonged to SF-1/Ad4BP group and that gfFTZ-F1 transcripts were only expressed in the gonads and kidney but not in other tissues.Likewise our data suggests that the gfFTZ-F1 gene may share similar functions with other fish and mammalian counterparts,though further study is needed to make any definitive conclusions. The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination. We isolated the homologue of FTZ-F1 in genetically improved farmed The full-length cDNA was isolated from the ovary, which included an open reading frame encoding predicted protein of 486 amino acids. Sequence, tissue distribution and phylogenic analysis of the FTZ-F1 showed that the gfFTZ- F1 belonged to SF-1 / Ad4BP group and that gfFTZ-F1 transcripts were only expressed in the gonads and kidney but not in other tissues.Likewise our data suggests that the the gfFTZ-F1 gene may share similar functions with other fish and mammalian counterparts, though further study is needed to make any definitive conclusions.
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