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大豆根瘤菌接种的增产效果常受土壤肥力及施氮水平的影响。本试验设置不同接种及施氮水平,旨在确定根瘤菌接种效应与氮肥用量的关系,为推广根瘤菌接种及配合施用氮肥提供依据。一、材料和方法采用盆栽试验。用土取自未种过大豆田的土壤,为沙质淡灰钙土,0~20厘米土层有机质为0.8%、含氮0.03%、含磷0.09%,水解氮35ppm,速效磷6.4ppm,含盐量0.03%,pH8.5。根瘤菌剂是辽宁兽医生物药品厂产品。大豆品种为铁丰18号。试验设两种接种水平(接种、不接种),三种施氮水平每盆施尿素0、1、2克,约折每亩0、24、48斤,组成六种处理,随机区组,重复六次(其中,一重复用于生长中期取样测定)。每盆种植大豆3株。
Soybean rhizobia vaccination yield effects often affected by soil fertility and nitrogen levels. The experiment set different inoculation and nitrogen application level, aimed at determining the relationship between inoculation effect of Rhizobium and the amount of nitrogen fertilizer, in order to promote Rhizobium inoculation and provide the basis for the application of nitrogen fertilizer. First, materials and methods Pot experiment. The soil was taken from the soil that had not been planted with soybeans. The soil of 0-20 cm soil layer was 0.8% of organic matter, 0.03% of nitrogen, 0.09% of phosphorus, 35 ppm of hydrolyzed nitrogen, 6.4 ppm of available phosphorus, Amount 0.03%, pH 8.5. Rhizobium agent is a veterinary biological medicine factory in Liaoning products. Soybean varieties for Iron Fung No. 18. Two levels of inoculation (inoculation and non-inoculation) were set up. Three nitrogen application rates were applied to 0, 1 and 2 grams of urea per pot, accounting for 0, 24 and 48 pounds per acre respectively. Six treatment groups were randomly assigned to repeat Six times (of which one was repeated for the mid-growth sampling assay). Soybean planted three pots per pot.