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目的扩增弓形虫主要表面抗原（P30）基因编码序列并进行重组表达方法设计合成引物，从弓形虫基因组DNA中扩增P30基因编码序列，克隆入载体pGEM-T和PGEX-4T-1，经PCR和酶切筛选，测序验证后，进行诱导表达和Westemblot鉴定结果从弓形虫基因组DNA中扩增出P30基因编码序列，并诱导表达出能被兔抗弓形虫血清识别的重组P30结论P30克隆载体和GST融合表达载体的构建和P30的成功表达，为进一步从基因水平和蛋白水平研究P30及进行诊断试剂和疫苗研究创造了条件。
Objective To amplify the coding sequence of the major surface antigen (P30) gene of Toxoplasma gondii and recombine it with the recombinant plasmid. The coding sequence of P30 gene was amplified from the genome DNA of Toxoplasma gondii and cloned into vector pGEM-T and PGEX-4T-1. PCR and restriction enzyme digestion. After sequencing validation, induced expression and Westemblot identification were performed. The coding sequence of P30 gene was amplified from Toxoplasma gondii genome DNA and the recombinant P30 gene was induced to be recognized by rabbit anti-Toxoplasma gondii. Conclusion The P30 cloning vector The construction of GST fusion expression vector and the successful expression of P30 have provided the conditions for the further study of P30 at gene and protein level and the research of diagnostic reagents and vaccines.