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目的:研究表明miRNAs在人类恶性肿瘤的发生发展过程中起着重要作用,本研究检测6种miRNAs:miR-98、let-7d、let-7g、miR-96、miR-182及miR-183在前列腺癌组织中的表达情况及其临床意义。方法:采用原位分子杂交方法,结合组织芯片技术分别检测38例BPH和52例前列腺癌中6种miRNAs的表达情况。并对前列腺癌组织中6种miRNA与Gleason评分,临床分期及6种miRNA间进行相关分析。结果:6种miRNAs中,与BPH组织相比,在前列腺癌组织中表达降低的为miR-98、let-7d、let-7g;而表达升高的为miR-96、miR-182及miR-183,差异均有统计学意义(P<0.05)。6种miRNAs的表达与前列腺癌的Gleason评分均相关(P<0.05),但与患者的年龄及血清PSA水平均无明显相关性(P>0.05)。其中miR-96和miR-182的表达与前列腺癌的临床分期均有相关性(P<0.05),miR-98和miR-96的表达均与肿瘤累及前列腺的叶数存在相关性(P<0.05)。另外,前列腺癌组织中,miR-96、miR-182及miR-183的表达彼此之间呈正相关(P<0.01,r分别为0.41,0.44),let-7d与let-7g的表达之间呈正相关(P<0.01,r=0.46),miR-98与let-7d及let-7g的表达之间呈正相关(P<0.05,r分别为0.31,0.34)。结论:miR-98、let-7d、let-7g、miR-96、miR-182及miR-183构成的miRNA表达谱在一定程度上反映了前列腺癌的生物学行为,可能作为前列腺癌早期诊断和预后评估的重要生物标记物。
AIM: The study indicates that miRNAs play an important role in the development of human malignant tumors. In this study, we examined six miRNAs: miR-98, let-7d, let-7g, miR-96, miR-182 and miR- Prostate cancer tissue and its clinical significance. Methods: In situ hybridization and tissue microarray were used to detect the expression of six miRNAs in 38 cases of BPH and 52 cases of prostate cancer. Six kinds of miRNAs in the prostate cancer tissues were analyzed with Gleason score, clinical stage and six miRNAs. Results: The expression of miR-98, let-7d and let-7g in the six types of miRNAs was lower than that in BPH tissues, but the expression of miR-96, miR-182 and miR- 183, the differences were statistically significant (P <0.05). The expression of six miRNAs was correlated with the Gleason score of prostate cancer (P <0.05), but not with age and serum PSA level (P> 0.05). The expression of miR-96 and miR-182 was correlated with the clinical stage of prostate cancer (P <0.05). The expressions of miR-98 and miR-96 were correlated with the number of prostatic adenocarcinoma (P <0.05 ). In addition, there was a positive correlation between the expression of miR-96, miR-182 and miR-183 in prostate cancer tissues (P <0.01, r = 0.41 and 0.44, respectively) (P <0.01, r = 0.46). There was a positive correlation between miR-98 expression and let-7g and let-7g expression (P <0.05, r = 0.31,0.34, respectively) Conclusion: The miRNA expression profiles of miR-98, let-7d, let-7g, miR-96, miR-182 and miR-183 may reflect the biological behavior of prostate cancer to a certain extent. Prognostic assessment of important biomarkers.