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初步建立人脉络膜黑色素瘤的miRNA表达谱,并探讨相应的miRNA在该疾病中的功能,为阐明脉络膜黑色素瘤发病机制寻求有效的治疗途径提供理论和实验依据.利用miRNA微芯片技术检测脉络膜黑色素瘤的has-miRNA表达谱,将4例人脉络膜黑色素瘤组织总RNA分别与4例健康人脉络膜组织对比,筛选has-miRNA表达情况.根据生物信息学的有关方法处理芯片实验数据,筛选出共同差异表达的候选has-miRNA.采用实时定量PCR的方法,验证各候选has-miRNA的表达情况.将芯片和RT-PCR两种方法结论一致的候选hsa-miRNA确定为有意义的共同差异表达has-miRNA.运用miRNA芯片技术,发现人脉络膜黑色素瘤组织中miRNA-20a,miRNA-106a,miRNA-17,miRNA-21,miRNA-34a明显上调表达,miRNA-145和miRNA-204明显下调表达.在4例脉络膜黑色素瘤中,miRNA-20a,miRNA-106a,miRNA-17,miRNA-21,miRNA-34a明显表达上调,miRNA-145和miRNA-204明显表达下调.脉络膜黑色素瘤有差异表达的miRNA为研究肿瘤的发生机制研究提供了诊断和治疗的新思路.
Preliminary establishment of human choroidal melanoma miRNA expression profiles and explore the corresponding miRNA function in the disease, in order to elucidate the pathogenesis of choroidal melanoma to seek effective treatment to provide theoretical and experimental basis.Using miRNA micro-chip detection of choroidal melanoma MiRNA expression profiles of 4 human choroidal melanoma tissues were compared with 4 choroidal tissue samples to screen for the expression of has-miRNA.According to the bioinformatics methods to deal with the chip experimental data, the common differences were screened Expression of the candidate has-miRNA.Quantitative real-time PCR method to verify the expression of the candidate has-miRNA.Chip and RT-PCR two methods conclude the same candidate hsa-miRNA as a meaningful common expression difference has- miRNA-miRNA-miRNA-miRNA-miRNA-miRNA miRNA-miRNA miRNA-miRNA miRNA-miRNA miRNA-miRNA miRNA- In choroidal melanoma, miRNA-20a, miRNA-106a, miRNA-17, miRNA-21 and miRNA-34a were upregulated while miRNA-145 and miRNA-204 were down- Differentially expressed miRNAs provide a new way of diagnosis and treatment for studying the mechanism of tumorigenesis.