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采用动态pH联接-扫集毛细管电泳法对化妆品中的迷迭香酸进行检测。用重力进样的方式,进样高度为15 cm的情况下,研究了硼砂浓度、pH、十二烷基硫酸钠(SDS)浓度、甲醇浓度、样品基体、进样时间、分离电压对富集与分离的影响。优化后的实验条件:15 mmol/L硼砂-45 mmol/L SDS(pH8.8)-15%甲醇为缓冲液,进样时间60s,分离电压16kV,样品中磷酸盐浓度10 mmol/L,样品基质pH 4.7。在上述条件下,迷迭香酸(RA)的线性回归方程式为y=539200ρ+53588(r=0.9985),线性范围为0.144～3.6μg/mL,检出限0.036μg/mL,迷迭香酸的回收率为92.5%～103%,相对标准偏差为2.5%。
The rosmarinic acid in cosmetics was tested by dynamic pH-sweep-capillary electrophoresis. Under the condition of sample injection height of 15 cm, the concentrations of borax, pH, sodium dodecyl sulfate (SDS), methanol concentration, sample matrix, injection time and separation voltage on the enrichment With the effect of separation. The optimized experimental conditions were as follows: 15 mmol / L borax -45 mmol / L SDS (pH8.8) -15% methanol as buffer, injection time 60 s, separation voltage 16 kV, phosphate concentration 10 mmol / Matrix pH 4.7. Under the above conditions, the linear regression equation of rosmarinic acid (RA) was y = 539200ρ + 53588 (r = 0.9985), the linear range was 0.144 ~ 3.6μg / mL with the detection limit of 0.036μg / mL. The recoveries were 92.5% ~ 103% with relative standard deviations of 2.5%.