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为了探索提取土壤DNA之前去除腐殖酸的方法,采用芬顿试剂对土壤样品进行预处理,结果表明芬顿试剂可以有效地去除土壤中的腐殖酸,未经处理和经芬顿试剂处理20~100 min的土壤,均可提取到接近23 kb大小的宏基因组DNA。16S rDNA PCR扩增表明,40~100 min处理土壤提取的DNA可以成功扩增到目的片段,而处理20 min的土壤不一定能扩增到目的片段,未经处理的土壤则不能扩增到目的片段。芬顿试剂可以用于土壤预处理以去除腐殖酸。
In order to explore the method of removing humic acid before soil DNA extraction, pretreatment of soil samples with Fenton reagent showed that Fenton reagent can effectively remove humic acid in soil, untreated and treated with Fenton reagent ~ 100 min of soil, can be extracted to nearly 23 kb size of metagenomic DNA. The 16S rDNA PCR showed that DNA extracted from the soil for 40-100 min could be successfully amplified into the target fragment, while the soil for 20 min could not be amplified to the target fragment, and the untreated soil could not be amplified Fragment. Fenton’s reagent can be used for soil pretreatment to remove humic acid.