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目的:探讨健脾清热化瘀中药对大鼠胃溃疡愈合质量及胃黏膜炎症反应的影响。方法:采用冰醋酸注射法制备大鼠胃溃疡模型,随机分为模型组、雷尼替丁组(西药组)、健脾中药组(中药A组)、清热中药组(中药B组)、化瘀中药组(中药C组)和健脾清热化瘀中药组(中药D组),设立正常对照组(空白组)。在光镜下观察大鼠溃疡指数、再生黏膜厚度、黏膜肌层缺损宽度及炎症细胞密度,应用ELLISA法检测血清及胃黏膜EGF及TNF-α含量。结果:健脾、清热、化瘀中药及其合方都能使再生黏膜厚度增加,溃疡指数、黏膜肌层缺损宽度及炎症细胞密度减少,与模型组比较有非常显著性差异(P<0.01),中药合方组疗效优于西药组(P<0.05或P<0.01)。模型组胃黏膜TNF-α水平较空白对照组明显增加(P<0.01),血清TNF-α水平也有一定程度的上升(P<0.05),健脾清热化瘀中药治疗组胃黏膜TNF-α水平明显低于模型组(P<0.01)。治疗组血清及胃黏膜EGF含量较模型组及空白组升高(P<0.05或P<0.01)。结论:健脾清热化瘀中药能减轻炎症细胞的浸润,抑制炎性细胞因子的释放,改善胃溃疡再生黏膜组织学成熟度和功能成熟度,从而提高胃溃疡愈合质量。
Objective: To investigate the effects of Chinese herbal medicine Jianpi Qingre Huayu on the quality of gastric ulcer healing and gastric mucosal inflammation in rats. Methods: The rats model of gastric ulcer was prepared by injection of glacial acetic acid. The model rats were randomly divided into model group, ranitidine group (western medicine group), Chinese medicine group (Chinese medicine group A), Chinese herb group (Chinese medicine group B) The Chinese medicine group (Chinese medicine group C) and the Chinese herbal medicine group (Chinese medicine group D) of Jianpi Qingre Huayu were set up, and the normal control group (blank group) was established. Under light microscope, the ulcer index, regenerated mucosa thickness, mucosal muscle defect width and inflammatory cell density were observed. The contents of EGF and TNF-α in serum and gastric mucosa were detected by ELLISA. Results: The effects of invigorating the spleen, clearing away heat and removing blood stasis and their combination on the thickness of regenerative mucosa increased, the ulcer index, the width of mucosal muscle defect and the density of inflammatory cells decreased significantly compared with the model group (P <0.01) , Chinese medicine group prescription is better than Western medicine group (P <0.05 or P <0.01). The level of TNF-α in gastric mucosa in model group was significantly higher than that in blank control group (P <0.01), and the level of TNF-α in serum was also increased to some extent (P <0.05) Significantly lower than the model group (P <0.01). The content of EGF in serum and gastric mucosa in treatment group was higher than that in model group and blank group (P <0.05 or P <0.01). Conclusion: The Chinese herbs of invigorating the spleen, clearing away heat and removing blood stasis can relieve the infiltration of inflammatory cells, inhibit the release of inflammatory cytokines, improve the histological maturation and maturation of regenerative mucosa of gastric ulcer, and improve the healing quality of gastric ulcer.