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目的 了解系统性红斑狼疮 (systemiclupuserythematosus,SLE)患者血浆一氧化氮 (nitricoxide ,NO)的水平及其与病情的关系。方法 用硝酸还原酶显色分光光度比色法测定 2 0例活动期及 15例非活动期SLE患者和 30例正常健康对照组血浆一氧化氮含量 ,比较其水平差异。同时对比观察SLE患者血清抗dsDNA抗体的含量并与NO做了相关分析。结果 正常对照组血浆NO水平为 (6 4.82± 11.2 1) μmol/L ,SLE活动组 ,非活动组NO水平分别为(144 .5 0± 2 6 .83) μmol/L和 (90 .12± 10 .2 7) μmol/L。SLE患者血浆NO水平与正常对照组比较差异非常显著 (P <0 .0 1) ,SLE活动组与非活动组比较亦呈显著性差异 (P <0 .0 5 ) ,SLE患者血浆NO与血清抗dsDNA抗体呈显著正相关 (P <0 .0 1)。结论 NO可能参与了SLE的发病过程并与SLE的活动性呈一致性。
Objective To investigate the level of plasma nitric oxide (NO) in patients with systemic lupus erythematosus (SLE) and its relationship with the disease. Methods Nitric acid reductase colorimetric spectrophotometry was used to determine the plasma levels of nitric oxide in 20 active and 15 inactive SLE patients and 30 healthy controls. Meanwhile, the content of anti-dsDNA antibody in serum of SLE patients was compared and analyzed with NO. Results The level of NO in normal control group was (4.22 ± 11.21) μmol / L, the levels of NO in SLE group and inactive group were (144.500 ± 26.83) μmol / L and (90.12 ± 10 .2 7) μmol / L. The level of NO in plasma of SLE patients was significantly lower than that of normal controls (P <0.01), but there was significant difference between SLE patients and non-active patients (P <0.05) Anti-dsDNA antibody was significantly correlated (P <0. 01). Conclusion NO may participate in the pathogenesis of SLE and be consistent with the activity of SLE.