建立了一套克级的制备型超临界流体色谱 ,采用全多孔空白硅胶为固定相 ,二氧化碳加极性携带剂及酸碱改性剂为三元流动相 ,在该设备上对黄酮类物质和生物碱混合物进行了分离 ,讨论了分离条件对分离组分产率和纯度的影响。 A set of gram-scale preparative supercritical fluid chromatography was established. The whole porous blank silica gel was used as the stationary phase. Carbon dioxide plus polar carrier and acid-base modifier were ternary mobile phase. Flavonoids and The mixture of alkaloids was separated and the influence of the separation conditions on the yield and purity of the separated components was discussed.