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为研究水曲柳糖基转移酶8家族(glycosyltransferases,FmGT8)基因结构特征及编码蛋白性质,揭示其时空表达模式以及对应拉处理的响应机制,在获得4个水曲柳FmGT8基因的基础上,应用生物信息学软件对Fm GT8及其编码蛋白进行详细的分析;选择5—9月的水曲柳叶子、木质部以及外皮样本,利用实时荧光定量PCR分析FmGT8基因的表达模式;对水曲柳分别进行1、3、7 d的应拉处理,分析Fm GT8基因转录表达对应拉处理的响应模式。结果显示:获得了3个FmGT8全长基因(FmGT8-1,FmGT8-2,FmGT8-3)和1个Fm GT8基因片段(Fm GT8-4),已上传至NCBI并获得登录号分别为KP307672、KP307673、KP307674、KP307675。FmGT8-1、FmGT8-2、FmGT8-3在木质部中表达量最高,Fm GT8-4在外皮中的表达量最高;FmGT8-1、FmGT8-2、FmGT8-3均在6月的相对表达量达到最高,Fm GT8-4在9月表达量最高。4个基因在应拉处理材料中的表达模式各不相同,但均对应拉处理有响应。FmGT8-1、FmGT8-2、FmGT8-4在应拉受力面的表达量最高,FmGT8-3在应压受力面表达量最高,说明FmGT8具有时空特异性。研究结果表明FmGT8家族基因可能参与了细胞壁与木质素的合成。
In order to study the structural characteristics of the glycosyltransferases 8 (FmGT8) gene and the nature of the encoded proteins, revealing the temporal and spatial expression pattern and the response mechanism of the corresponding pull treatment, four FmGT8 genes were obtained, Fm GT8 and its encoded protein were analyzed in detail using bioinformatics software. The leaves, xylem and sheath samples from May to September were collected, and the expression pattern of FmGT8 gene was analyzed by real-time fluorescence quantitative PCR. 1, 3, 7 d pull-out treatment, analysis of Fm GT8 gene transcription response to pull the treatment response mode. The results showed that three full-length FmGT8 genes (FmGT8-1, FmGT8-2 and FmGT8-3) and one Fm GT8-4 gene fragment (Fm GT8-4) were obtained and uploaded to NCBI. The accession numbers were KP307672, KP307673, KP307674, KP307675. FmGT8-1, FmGT8-2 and FmGT8-3 had the highest expression in xylem, and Fm GT8-4 had the highest expression in the coat. The relative expression of FmGT8-1, FmGT8-2 and FmGT8-3 reached the highest in June Highest, Fm GT8-4 highest expression in September. The expression pattern of the four genes in different materials should be different, but they all respond to pull treatment. FmGT8-1, FmGT8-2 and FmGT8-4 had the highest expression in the stress-bearing plane, and FmGT8-3 had the highest expression in the stress-stressed plane, indicating that the FmGT8 has the space-time specificity. The results show that FmGT8 family of genes may be involved in the cell wall and lignin synthesis.