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目的研究胶质母细胞瘤(GBM)组织照射60Gy后基因表达谱中免疫相关基因表达差异的变化及意义。方法采用BioStarH-141s(2004)型含13929条人类全长基因cDNA表达谱芯片,对两例胶质母细胞瘤组织进行放疗前及放疗60Gy后检测,并分析它们之间的基因表达差异。结果胶质母细胞瘤放疗60Gy后与放疗前比较,表达差异基因中改变最明显的功能群是免疫系统相关的基因,如IGLV1-44、SLPI、CXCL14等上调。细胞增殖、细胞调亡、细胞周期、DNA修复系统也有部分基因发生明显变化,如MLL5上调、POLR2B下调等。结论提示对胶质母细胞瘤组织照射60Gy后基因表达谱改变的研究可以更好地阐明放射敏感性差异机理。
Objective To study the changes and significance of the expression of immune-related genes in gene expression profiles of glioblastoma (GBM) tissues irradiated with 60 Gy. METHODS: BioStarH-141s (2004) containing 13,929 human full-length cDNA microarray was used to detect the expression of gene in two glioblastoma tissues before radiotherapy and 60 Gy after radiotherapy. Results Glioblastoma 60Gy after radiotherapy compared with before radiotherapy, the expression of the most obvious change in the genes of the functional groups are immune system-related genes, such as IGLV1-44, SLPI, CXCL14 upregulation. Some genes such as MLL5 up-regulation and POLR2B down-regulation have also been observed in cell proliferation, apoptosis, cell cycle and DNA repair system. The conclusion suggests that the study of gene expression profile after 60Gy irradiation on glioblastoma tissue can better elucidate the mechanism of radiosensitivity difference.