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应用机械式弹性拉力打击器,以10.0m/s速度冲击犬胸骨心前区,建立免疫组织化学染色对伤后2~5分钟、30~60分钟、3小时及5小时心肌挫伤组织中Mb、CK-BB及CK-MM的变化进行观察。结果表明:(1)心肌挫伤后2~5分钟检出心内膜下肌层已有重度心肌Mb脱失及中度CK-BB、CK-MM脱失,并沉积于心肌纤维间,同时吸收入血。随着伤后时间延长,挫伤区三种物质脱失加重,但间质中沉积量减少乃至消失;(2)波浪变心肌中也可见三种物质的轻度脱失;(3)同一心脏中不同部位心肌的损伤程度不同、证明应用免疫组织化学染色技术可发现心肌挫伤性损害。心前区受到相当程度的钝力冲击后心脏不同部位损害程度不同。
A mechanical elastic tensioner was used to impact the precancerous area of the canine sternum at a speed of 10.0m / s. Immunohistochemical staining was performed to detect the expression of Mb in myocardial contusion tissues at 2 to 5 minutes, 30 to 60 minutes, 3 hours and 5 hours after injury , CK-BB and CK-MM were observed. The results showed that: (1) Myocardial submucosal muscular layer has been found to have severe myocardial Mb loss and moderate CK-BB, CK-MM loss 2 ~ 5 minutes after myocardial contusion, and deposited in myocardial fibers, while absorbing Into the blood. With the extension of time after injury, three substances in the contusion zone were exacerbated, but the deposition in the stroma decreased or even disappeared. (2) Mild loss of three substances was seen in the myocardium of the wavy heart. (3) In the same heart Different parts of the degree of myocardial injury is different, that the use of immunohistochemical staining can be found in contusion injury. Precordial area by a considerable degree of blunt impact of different parts of the heart damage to varying degrees.