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目的:研究AAV9-SERCA 2a基因转染对急性心房颤动(房颤)动物模型心房肌细胞上L型钙通道表达的影响。方法:采用60只新西兰大白兔为实验对象,采用心包注射法转染AAV9-SERCA 2a基因;将实验对象随机分为4组:房颤组、转荧光蛋白房颤组、转SERCA 2a基因房颤组和对照组。转染后建立急性房颤模型,采用膜片钳及荧光免疫组织化学法检测心房肌细胞上L型钙通道的电流变化及其L型钙通道蛋白表达的情况。结果:转SERCA 2a基因房颤组心房肌细胞上LVDCCa1c蛋白表达明显高于房颤组(P<0.05),与对照组比较差异无统计学意义。转SERCA 2a基因房颤组心房肌细胞上L型钙通道电流密度高于房颤组,差异有统计学意义(P<0.05),与对照组比较差异无统计学意义。结论:转SERCA 2a基因治疗可减少急性房颤模型心房肌细胞钙超载,减少房颤对L型钙通道电流密度的影响,对防止心脏电重构具有积极的意义。
Objective: To investigate the effect of AAV9-SERCA2a gene transfection on the expression of L-type calcium channel in atrial myocytes of acute atrial fibrillation (AF) animal model. Methods: A total of 60 New Zealand white rabbits were used as experimental subjects, and the AAV9-SERCA2a gene was transfected by pericardial injection. The subjects were randomly divided into 4 groups: atrial fibrillation group, fluorogenic AF group, SERCA2a gene AF group Group and control group. The model of acute atrial fibrillation was established after transfection. The changes of L-type calcium channel current and the expression of L-type calcium channel in atrial myocytes were detected by patch clamp and fluorescence immunohistochemistry. Results: The expression of LVDCCa1c in atrial fibrillation group with SERCA2a gene was significantly higher than that in atrial fibrillation group (P <0.05). There was no significant difference between the two groups. The current density of L-type calcium channel in atrial fibrillation group with SERCA 2a gene was higher than that of atrial fibrillation group (P <0.05), and there was no significant difference between the two groups. CONCLUSION: SERCA 2a gene therapy can reduce the calcium overload of atrial fibrillation in acute atrial fibrillation model and reduce the influence of atrial fibrillation on L-type calcium channel current density, which has a positive effect on preventing cardiac electrical remodeling.