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目的探讨内皮抑素对实体肿瘤血管生成的抑制作用。方法将60只小鼠成功建立肉瘤移植瘤后随机均分为内皮抑素5mg·kg~(-1)·d~(-1)治疗组(A1组)、内皮抑素10mg·kg~(-1)·d~(-1)治疗组(A2组)、内皮抑素15mg·kg~(-1)·d~(-1)治疗组(A3组)和生理盐水对照组(B组)。记录肿瘤生长情况,荧光显微镜下观察灌注血管距离,免疫组织化学染色观察肿瘤微血管密度(MVD)。结果与B组相比,A1、A2、A3组呈浓度依赖性地抑制肿瘤生长(P<0.05),肿瘤生长抑制率分别为24.91%、45.83%、65.30%。与B组相比,A1、A2、A3组灌注血管距离逐渐增大[(66.06±14.98)μm、(84.94±6.99)μm、(108.16±18.10)μm vs.(43.33±8.21)μm](P<0.05),而MVD逐渐减少[(30.27±9.95)个/400Hp、(17.87±7.69)个/400Hp、(9.60±4.31)个/400Hp vs.(43.13±9.86)个/400Hp](P<0.05)。结论内皮抑素可呈浓度依赖性地抑制肿瘤血管生成,从而抑制肿瘤生长,有望成为实体肿瘤临床治疗的有效药物。
Objective To investigate the inhibitory effect of endostatin on solid tumor angiogenesis. Methods Sixty mice were randomly divided into 3 groups: group treated with endostatin 5 mg · kg -1 · d -1 (group A1), endostatin 10 mg · kg -1 1) · d -1 group (A2), 15 mg · kg -1 · d -1 group (group A3) and saline control group (group B). The growth of tumor was recorded. The distance of perfusion vessel was observed under fluorescence microscope. MVD was observed by immunohistochemical staining. Results Compared with group B, A1, A2 and A3 inhibited tumor growth in a concentration - dependent manner (P <0.05). The tumor growth inhibition rates were 24.91%, 45.83% and 65.30%, respectively. Compared with group B, the distance of blood perfusion in group A1, A2 and A3 increased gradually ([(66.06 ± 14.98) μm, (84.94 ± 6.99) μm, (108.16 ± 18.10) μm vs. (43.33 ± 8.21) μm] <0.05), while MVD gradually decreased (30.27 ± 9.95) /400Hp, (17.87 ± 7.69) /400Hp, (9.6 ± 0.4.31) / 400Hp vs. (43.13 ± 9.86) /400Hp](P<0.05 ). Conclusion Endostatin can inhibit tumor angiogenesis in a concentration-dependent manner, thereby inhibiting tumor growth and is expected to become an effective drug for the clinical treatment of solid tumors.