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目的:建立高效液相色谱法测定脑脉泰胶囊中银杏总黄酮含量的方法。方法:色谱柱:伊利特-C_(18)柱(250mm×4.6 mm,5μm),流动相:乙腈-甲醇-水(9:39:52),磷酸调节pH至4.0,流速:0.8ml·min~(-1),检测波长:370nm,柱温:35℃。结果:槲皮素、山奈素、异鼠李素检测浓度的线性范围分别为4.20～25.20(r=0.999 7),4.85～29.10(r=0.999 5),2.02～12.12μg·ml~(-1)(r=0.999 2);平均回收率分别为99.90%,100.10%,98.33%,RSD分别为0.63%,0.80%,0.99%。结论:本法快速、简便、准确、重复性好,可用于脑脉泰胶囊的质量控制。
Objective: To establish a method for the determination of total flavonoids in Gingko biloba by Naotai capsule. METHODS: Column: Ilite-C_(18) column (250 mm×4.6 mm, 5 μm), mobile phase: acetonitrile-methanol-water (9:39:52), phosphoric acid to adjust pH to 4.0, flow rate: 0.8 ml·min ~(-1), detection wavelength: 370 nm, column temperature: 35°C. Results: The linear ranges of quercetin, kaempferol and isorhamnetin were 4.20-25.20(r=0.999 7), 4.85-29.10(r=0.999 5), 2.02-12.12μg·ml-1. (r=0.999 2); The average recoveries were 99.90%, 100.10%, and 98.33%, respectively, and the RSDs were 0.63%, 0.80%, and 0.99%, respectively. Conclusion: This method is rapid, simple, accurate and reproducible, and can be used for the quality control of Naomaitai Capsule.