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大针茅(Stipa grandis)是我国北方典型草原上最重要的饲用植物之一,分布广,数量大,是优势种之一。本研究应用RAPD技术检测大针茅植物不同放牧压力下群体的遗传多样性,探索放牧强度与遗传分化的相互联系。在放牧和对照群体中,利用18个有效引物共获得了129个位点,其中多态性位点为105个,占81.40%,说明个体发生较高的遗传变异。两个群体都具有特异性位点,说明放牧压力导致某些位点发生了变化,但遗传变异主要存在于群体内(遗传一致度为90.43%)。
Stipa grandis is one of the most important forages in the typical steppe of northern China. It is widely distributed and large in number and is one of dominant species. In this study, RAPD technique was used to detect the genetic diversity of populations under different grazing pressures of Stipa grandis plants and to explore the interrelationship between grazing intensity and genetic differentiation. In the grazing and control groups, a total of 129 loci were obtained using 18 effective primers, of which 105 were polymorphic, accounting for 81.40% of the total, indicating that individuals had higher genetic variation. Both groups had specific loci, indicating that grazing pressure caused certain sites to change, but genetic variation mainly existed in the population (genetic identity was 90.43%).