采用微波对Vc二步发酵伴生菌苏云金芽孢杆菌进行照射,然后将其涂布到含有15mg/mL吖啶橙的平板中。经过微波和吖啶橙的复合诱变作用,筛选到一株高效伴生菌F328。在pH6.8的发酵培养基中与氧化葡萄糖酸杆菌混合发酵,糖酸转化率提高了7.77%,达到91.55%,2-酮基-L-古龙酸的质量浓度达到90.2mg/mL。菌株F328经过连续传代5次,遗传性状稳定。 The Vc two-step fermentative companion Bacillus thuringiensis was irradiated with microwaves and then plated onto plates containing acridine orange at 15 mg / mL. After microwave and acridine orange compound mutagenesis, a highly efficient companion F328 was screened. Mixed fermentation with Gluconobacter oxydans in fermentation medium pH6.8, the conversion rate of sugar and acid increased by 7.77% and reached 91.55%, and the mass concentration of 2-keto-L-gulonic acid reached 90.2 mg / mL. Strain F328 after 5 consecutive passages, genetic stability.