论文部分内容阅读
目的 观察不同剂量、不同培养时间的水镁石对大肠埃希菌生长、代谢产物及酶的影响。方法 测定细菌数量、培养基pH值和Mg2 + 、葡萄糖、丙酮酸的含量及 β -半乳糖苷酶活力的变化。结果 随着水镁石浓度及培养时间的增加 ,葡萄糖含量逐渐下降 ,而培养基pH值、细菌数量和Mg2 + 、丙酮酸的含量以及 β -半乳糖苷酶活力逐渐增加。当水镁石浓度为1 6 0 0 μg/ml 培养 1 2h以及 80 0 μg/ml 培养2 4h ,各指标差异均有显著性。结论 水镁石能促进大肠埃希菌的生长代谢。
Objective To observe the effect of brucite with different dosages and incubation time on the growth, metabolites and enzymes of Escherichia coli. Methods The changes of bacteria quantity, pH value, Mg2 +, glucose and pyruvate contents and β - galactosidase activity were measured. Results With the increase of brucite concentration and incubation time, the content of glucose decreased gradually, but the pH value, bacteria quantity, Mg2 +, pyruvic acid content and β - galactosidase activity increased gradually. When the brucite concentration of 1600μg / ml cultured 1 2h and 80 0 μg / ml cultured 2 4h, the differences were significant in all indicators. Conclusion Brucite can promote the growth and metabolism of Escherichia coli.