论文部分内容阅读
目的探讨2000μW/cm2电磁辐射对大鼠海马N-甲基-D-门冬氨酸受体亚单位2B(N-methy1-D-asparaterecep-torsubunit2B,NR2B)蛋白及其mRNA水平表达的影响,揭示电磁辐射对大鼠学习记忆功能的损伤机制。方法实验分为空白对照组,假辐射组,1h/d、2h/d、3h/d辐射组。将辐射组大鼠固定体位,头部接受功率密度为2000μW/cm2的近场辐射,连续辐射30d。通过Morris水迷宫检测大鼠的空间学习记忆能力,采用免疫组化法和Western-Blot法检测大鼠海马组织NR2B蛋白表达的变化,RT-PCR法检测大鼠海马组织NR2BmRNA表达的变化。结果假辐射组大鼠各项指标与空白对照组相比差异均无统计学意义(P﹥0.05);而各辐射组大鼠寻找安全平台的逃避潜伏期较空白对照组明显延长(P﹤0.05);各辐射组大鼠海马神经元排列紊乱,NR2B阳性细胞比率显著下降,海马组织NR2B蛋白及其mRNA表达水平明显降低(P﹤0.05)。结论 2000μW/cm2电磁辐射可导致大鼠学习记忆功能下降,其机制可能与大鼠海马组织NR2B蛋白及其mRNA的表达降低有关。
Objective To investigate the effect of 2000μW / cm2 electromagnetic radiation on the expression of N-methy1-D-asparaterecep-torsubunit2B (NR2B) protein in rat hippocampus Damage Mechanism of Electromagnetic Radiation on Learning and Memory in Rats. Methods The experiment was divided into blank control group, sham radiation group, 1h / d, 2h / d, 3h / d radiation group. Radiation rats were fixed position, the head to receive power density of 2000μW / cm2 near-field radiation, continuous irradiation 30d. The spatial learning and memory abilities of rats were detected by Morris water maze. The expression of NR2B protein in hippocampus was detected by immunohistochemistry and Western-Blot. The expression of NR2B mRNA in hippocampus was detected by RT-PCR. Results There was no significant difference between sham radiation group and blank control group (P> 0.05), while the escape latency of rats in each radiation group was significantly longer than that of blank control group (P <0.05) The neurons in hippocampus were disordered and the ratio of NR2B positive cells decreased significantly in hippocampus. The expression of NR2B protein and mRNA in hippocampus were significantly decreased (P <0.05). Conclusion 2000μW / cm2 electromagnetic radiation can lead to decreased learning and memory in rats, which may be related to the decreased expression of NR2B protein and mRNA in the hippocampus of rats.