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目的了解斜纹夜蛾核型多角体病毒的小鼠急性致病性。方法参照《微生物农药急性致病性试验方法》(试行)1998和美国EPA微生物农药试验准则(OPPTS 885.3200)进行。SPF级NIH小鼠随机分为3组,500 mg/kg(3×108PIB/20 g)和16.7 mg/kg(1×107PIB/20 g)染毒组每组40只,生理盐水对照组20只,雌雄各半。结果在本实验条件下,斜纹夜蛾核型多角体病毒在500 mg/kg剂量对NIH小鼠具有强致病性,靶器官为脑、肝脏、肺脏和脾脏;在16.7 mg/kg剂量未发现明显致病性。结论斜纹夜蛾核型多角体病毒小鼠急性致病性的评价结果与应用的剂量有关,应制定合理的国家标准评价微生物农药的小鼠急性致病性。
Objective To understand the acute pathogenicity of Spodoptera litura nucleopolyhedrovirus in mice. The method is based on the “Microbiological Pesticide Acute Pathogenicity Test Method” (Trial) 1998 and the United States EPA Microbial Pesticide Test Guidelines (OPPTS 885.3200). SPF NIH mice were randomly divided into 3 groups: 40 mg / kg (3 × 108 PIB / 20 g) and 16.7 mg / kg (1 × 107 PIB / 20 g) , Male and female half. Results Under the experimental conditions, S. litura NPV showed strong pathogenicity to NIH mice at the dose of 500 mg / kg, and the target organs were brain, liver, lung and spleen at the dose of 16.7 mg / kg Obviously pathogenic. Conclusion The evaluation of the acute pathogenicity of Spodoptera litura nucleopolyhedrovirus in mice is related to the dose applied. Appropriate national standards should be developed to evaluate the acute pathogenicity of mice in the presence of microbial pesticides.