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目的建立弱阳离子交换(WCX)固相萃取法提取血浆中13种主要作用于中枢神经系统的药物并用高效液相色谱-二极管阵列检测器(HPLC-DAD)分析。方法血浆经WCX小柱固相萃取后,在Agilent TC-C18色谱柱上,以磷酸盐缓冲液-乙腈为流动相,采用梯度洗脱,流速为1.5 mL.min-1,扫描波长:200~364 nm;检测波长:210 nm。结果 13种药物的萃取回收率72.60%~110.24%,精密度均小于11.55%,检测限0.01~0.10 mg.L-1,线性相关系数在0.998 5以上。结论本法快速、灵敏、重现性好,可用于同时分析测定血浆中的13种药物。
Objective To establish a weak cation exchange (WCX) solid-phase extraction method for the extraction of thirteen drugs in the central nervous system in plasma and analyze by HPLC-DAD (High Performance Liquid Chromatography-Diode Array Detector). Methods The plasma was purified by WCX column and eluted with gradient elution on a Agilent TC-C18 column using phosphate buffer-acetonitrile as mobile phase with a flow rate of 1.5 mL · min-1 and a scan wavelength of 200 ~ 364 nm; detection wavelength: 210 nm. Results The recoveries of 13 drugs ranged from 72.60% to 110.24%. The precision was less than 11.55%. The detection limit was 0.01-0.10 mg.L-1. The linear correlation coefficient was above 0.998 5. Conclusion The method is rapid, sensitive and reproducible. It can be used for the simultaneous analysis and determination of 13 drugs in plasma.