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目的:研究肉桂多酚改善HepG2细胞胰岛素抵抗的分子机制。方法:以HepG2细胞为模型,设立对照组和肉桂多酚实验组,肉桂多酚组设5,10,15 mg.L-13种不同质量浓度组。肉桂多酚作用细胞24 h后,运用实时定量聚合酶链反应(RT-PCR)技术,检测肉桂多酚对胰岛素抵抗HepG2细胞内葡萄糖转运蛋白2(GLUT2)、磷酸烯醇丙酮酸羧激酶(PEPCK)和葡萄糖-6-磷酸酶(G-6-Pase)基因表达的影响。结果:与对照组比较,肉桂多酚作用细胞24 h后,降低了GLUT2 mRNA的表达(P<0.05);肉桂多酚能够明显降低PEPCK,G-6-Pase mRNA的表达(P<0.05,P<0.01),且随着浓度的升高,肉桂多酚对PEPCK和G-6-Pase mRNA表达的抑制作用越明显。结论:肉桂多酚对HepG2胰岛素抵抗具有明显的改善作用,其机制可能与降低细胞内GLUT2,PEPCK和G-6-Pase mRNA的表达有关。
Objective: To study the molecular mechanism of cinnamon polyphenols in improving insulin resistance in HepG2 cells. Methods: HepG2 cells were used as a model to establish 5,10,15 mg.L-13 different concentration groups of control and cinnamon polyphenols experimental groups and cinnamon polyphenol groups. Cinnai polyphenol treated cells for 24 h, using real-time quantitative polymerase chain reaction (RT-PCR) technology to detect the effects of cinnamon polyphenols on insulin resistance in HepG2 cells glucose transporter 2 (GLUT2), phosphoenolpyruvate carboxykinase (PEPCK ) And glucose-6-phosphatase (G-6-Pase) gene expression. Results: Compared with the control group, cinnamon polyphenols decreased the expression of GLUT2 mRNA (P <0.05) 24 h after treatment with cinnamon polyphenols; cinnamyl polyphenols decreased the expression of PEPCK and G-6-Pase mRNA significantly (P <0.05, P <0.01). With the increase of concentration, the inhibitory effect of cinnamon polyphenols on the expression of PEPCK and G-6-Pase mRNA was more obvious. CONCLUSION: Cinnamon polyphenols can significantly improve the insulin resistance of HepG2 cells. The mechanism may be related to the decrease of intracellular GLUT2, PEPCK and G-6-Pase mRNA expression.