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目的建立陕西产黄芩HPLC指纹图谱,为后期黄芩研究指纹图谱质量控制提供依据。方法采用dimak C18柱,流动相为乙腈-0.1%磷酸水溶液线性梯度洗脱,检测波长为280 nm。结果运用梯度洗脱能很好分离黄芩的各类成分,通过软件比较,黄芩药材的指纹图谱相似度均大于0.85。结论本研究所建立的指纹图谱可作为黄芩药材的鉴定方法和陕产黄芩药材实验依据。
Objective To establish a HPLC fingerprint of Scutellaria baicalensis in Shaanxi Province and provide evidence for the research on the quality control of Scutellaria baicalensis in fingerprinting. Methods Dimak C18 column was used. The mobile phase consisted of a linear gradient of acetonitrile-0.1% phosphoric acid. The detection wavelength was 280 nm. Results Gradient elution could be used to separate various components of Scutellaria baicalensis Georgi. The similarity of fingerprints of Scutellaria baicalensis Georgi was greater than 0.85 by software comparison. Conclusion The fingerprinting established in this study can be used as the identification method of Radix Scutellariae and experimental basis of Radix Scutellariae.