论文部分内容阅读
目的:研究组胺H1受体拮抗剂——氮卓斯汀(azelastine,AZT)对豚鼠哮喘的拮抗作用,探讨氮卓斯汀拮抗哮喘的可能机制。方法:以卵白蛋白致敏豚鼠,制备哮喘模型,通过豚鼠引喘潜伏期和跌倒率的变化评价氮卓斯汀对哮喘的拮抗作用;分别计数外周血和支气管肺泡灌洗液(BALF)中嗜酸性粒细胞的数量;采用ELISA法检测血清和BALF中IL-4和IL-5的质量浓度。结果:与模型组相比,氮卓斯汀能显著延长致敏豚鼠的引喘潜伏期,降低跌倒率;氮卓斯汀显著减少哮喘豚鼠血清和BALF中嗜酸性粒细胞的数量,降低血清和BALF中IL-4和IL-5的水平。结论:降低IL-4和IL-5的水平,减少血中嗜酸性粒细胞的数量及其在气道中的浸润,可能是组胺H1受体拮抗剂——氮卓斯汀拮抗哮喘的机制之一。
AIM: To investigate the antagonism of histamine H1 antagonist azaserine (AZT) on asthma in guinea pigs and to explore the possible mechanism of azelastine antagonizing asthma. Methods: Guinea pigs were sensitized with ovalbumin to prepare asthmatic model. The antagonistic effect of azelastine on asthma was evaluated by the change of latent period and fall rate of guinea pig induced asthma. The eosinophilicity in peripheral blood and bronchoalveolar lavage fluid (BALF) The number of granulocytes was measured. The concentrations of IL-4 and IL-5 in serum and BALF were detected by ELISA. Results: Compared with the model group, azelastine could significantly prolong the latent period of asthma in sensitized guinea pigs and decrease the fall rate. Azelastine significantly reduced the number of eosinophils in serum and BALF of asthmatic guinea pigs and decreased the serum and BALF In IL-4 and IL-5 levels. CONCLUSIONS: The mechanism of antagonizing asthma by histamine H1 receptor antagonist-azelastine may be decreased by decreasing the levels of IL-4 and IL-5, decreasing the number of blood eosinophils and infiltration in the airway one.