研究双丁酰环磷酸腺苷（ｄｂｃＡＭＰ）和全反式维甲酸（ＡＴＲＡ）两种分化诱导剂对人肝癌细胞株ＳＭＭＣ－７７２１亚细胞组分中酪氨酸蛋白激酶（ＴＰＫ）的早期（２４ｈ内）效应。方法用超离心等法制备胞液（ｃ），胞核（ｎ）和膜性（ｍ）ＴＰＫ酶液，以聚谷氨酸∶酪氨酸（ｐｏ１ｙＥ．Ｙ）４∶ｌ及γ－３２Ｐ－ＡＴＰ为底物测定ＴＰＫ活力。结果对照和ｄｂｃＡＭＰ处理１ｈ使ｃＴＰＫ和ｎＴＰＫ升高，以后对照降至原有水平，而ｄｂｃＡＭＰ处理细胞的ｎＴＰＫ在１２～２４ｈ略低于对照细胞，但ｍＴＰＫ在１ｈ反而降低，在３ｈ（对照）或６ｈ（ｄｂｃＡＭＰ）升至高峰，１２ｈ后ｄｂｃＡＭＰ使ｍＴＰＫ活力低于对照。ＡＴＲＡ作用于ＳＭＭＣ－７７２１细胞后，１ｈ可使三类ＴＰＫ活力均升至高峰，１２ｈ后ｃＴＰＫ和ｎＴＰＫ活力低于对照细胞，但ｍＴＰＫ活力在２４ｈ才低于对照。结论ｄｂｃＡＭＰ和ＡＴＲＡ对不同亚细胞组分ＴＰＫ有不同的时相效应。一般说来，早期（１～６ｈ）有升高作用，而在１２～２４ｈ则有一下降作用，呈现双相效应 To investigate the effects of dbcAMP and all-trans retinoic acid (ATRA) on differentiation of tyrosine kinase (TPK) in human hepatocellular carcinoma cell line SMMC-7721 Within) effect. Methods Cytoplasm (c), nucleus (n) and membranous (m ) TPK enzyme solution were prepared by ultracentrifugation method with polyglutamic acid: tyrosine (po1yE.Y) 4: 1 and γ -32P-ATP as a substrate for the determination of TPK activity. The results of control and db cAMP 1h so that cTPK and nTPK increased after the control dropped to the original level, and db cAMP cells treated nTPK at 12 ~ 24h slightly lower than the control cells, but m TPK in 1h but decreased at 3h (control) or 6h (db cAMP) rose to peak, 12h after db cAMP so that m TPTP activity was lower than the control. After treated with ATRA in SMMC-7721 cells, the activity of all three TPKs peaked at 1h. After 12h, the activity of c-TPK and n-TPK was lower than that of control cells, but the activity of m-TPK was lower than that of the control after 24h. Conclusion db  cAMP and ATRA on different subcellular components TPK have different phase effects. In general, the early (1 ~ 6h) increased role, while in 12 ~ 24h there is a decline, showing a two-phase effect