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目的:了解和揭示便可通对SD大鼠烫伤后1周内血浆肿瘤坏死因子、内毒素、肠组织细胞凋亡的影响,观察烫伤大鼠小肠上皮细胞超微结构的变化,探讨本药对肠黏膜屏障保护作用机制。方法:将SD大鼠70只随机分成3组,第1组为中药治疗组(便可通组)30只;第2组为对照组(单纯烫伤组)30只,制备成烫伤模型,给予生理盐水灌胃;第3组为正常组10只。治疗组和对照组在给药后第1、3、7天各处死10只,均检测血浆肿瘤坏死因子、内毒素的含量,观察大鼠肠组织细胞凋亡的变化,给药后第7天观察大鼠小肠上皮细胞超微结构变化。结果:便可通可明显抑制烫伤大鼠内毒素易位,减少肿瘤坏死因子的产生,抑制肠组织细胞凋亡,明显修复和改善烫伤大鼠肠黏膜细胞超微结构的损伤。结论:中医复方便可通有较理想的肠道屏障保护作用,是预防和治疗肠道屏障功能障碍引发的多器官、多系统功能衰竭的有效的药物。
Objective: To understand and reveal the effect of Tongketong on plasma tumor necrosis factor, endotoxin and intestinal cell apoptosis in scald rats 1 week after scald, observe ultrastructural changes of small intestine epithelial cells in scald rats, and explore Intestinal mucosal barrier protective mechanism. Methods: 70 SD rats were randomly divided into 3 groups. The first group consisted of 30 Chinese medicine treatment groups (Tai Tong pass group) and the second group consisted of 30 control groups (only scald group). The scald model was prepared and the physiology was given. Saline was administered by gavage; group 3 was normal group. In the treatment group and the control group, 10 rats were sacrificed on the 1st, 3rd and 7th day after administration. The contents of plasma tumor necrosis factor and endotoxin were measured. The changes of apoptosis in the intestine of the rats were observed, and the 7th day after administration was observed. Observed ultrastructural changes in rat intestinal epithelial cells. RESULTS: It could significantly inhibit the endotoxin translocation in scald rats, reduce the production of tumor necrosis factor, inhibit the apoptosis of intestinal tissue, and obviously repair and improve the damage of ultrastructure of intestinal mucosa cells in burned rats. Conclusion: The reconvenience of traditional Chinese medicine can be used to protect the intestinal barrier, which is an effective drug for prevention and treatment of multi-organ and multi-system functional failure caused by intestinal barrier dysfunction.