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目的探讨Th17、CD4+CD25+调节性T(Treg)细胞及其相关细胞因子在子痫前期中的表达及在其发病过程中的作用。方法选取子痫前期患者30例的胎盘组织及正常妊娠25例的胎盘组织(对照组),采用实时荧光定量PCR法检测胎盘组织中Treg细胞转录因子Foxp3和与Th17表达相关的细胞因子孤儿核受体RORc、IL-17及与调节Th17、Treg细胞发育和功能相关的IL-23、IL-27及IL-27受体mRNA的表达量。结果子痫前期组胎盘组织中Treg细胞转录因子Foxp3表达较对照组明显降低,差异有统计学意义(P<0.05);子痫前期组胎盘组织中与Th17表达相关的细胞因子RORc、IL-17表达与对照组相比明显升高,差异有统计学意义(P<0.05);而与对照组相比,子痫前期组胎盘组织中IL-23、IL-27、IL-27受体表达也明显升高,差异均有统计学意义(P<0.05)。结论 Th17、Treg细胞及其相关细胞因子的表达在子痫前期中均有明显变化,可能参与了子痫前期的发病过程。
Objective To investigate the expression of Th17, CD4 + CD25 + regulatory T (Treg) cells and related cytokines in preeclampsia and their role in the pathogenesis. Methods The placental tissues from 30 patients with preeclampsia and the placenta from 25 normal pregnant women (control group) were collected. The transcription factor Foxp3 and the orphan nuclear receptor related to Th17 in placenta were detected by real-time fluorescence quantitative PCR Body RORc, IL-17 and the expression of IL-23, IL-27 and IL-27 receptor mRNAs associated with the regulation of Th17, Treg cell development and function. Results The expression of Foxp3 in placenta of preeclampsia was significantly lower than that in control group (P <0.05). The levels of cytokines RORc and IL-17 in placenta of preeclampsia Compared with control group, the expression of IL-23, IL-27 and IL-27 in placenta of preeclampsia group was significantly higher than that in control group (P <0.05) Significantly higher, the difference was statistically significant (P <0.05). Conclusion The expression of Th17 and Treg cells and their related cytokines are obviously changed in preeclampsia and may be involved in the pathogenesis of preeclampsia.