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本工作采用显微测量、图像分析和流式细胞分析方法，研究了细胞因子ｒｈＩＬ—１β、ｒｈＩＬ－２及ｒｈＩＬ—６对新生大鼠海马培养神经元突起生长发育、胞体生长状态、神经元存活数及培养过程中蛋白总量影响。结果显示，这３种细胞因子均对海马培养神经元的突起生长，胞体发育以及发育过程中神经元的蛋白质合成具有明显的促进作用，并且在提高海马培养神经元存活、延长存活时间方面有较为显著的效应。提示白介素类细胞因子ｒｈＩＬ—１β、ｒｈＩＬ－２及ｒｈＩＬ—６对海马神经元具有营养作用。
In this study, the effects of rhIL-1β, rhIL-2 and rhIL-6 on neurite outgrowth, cell growth, neuron survival in hippocampus of neonatal rats were studied using microscopic measurement, image analysis and flow cytometry Number and total protein during culture. The results showed that all of the three cytokines promoted neurite outgrowth, somatic cell development and neuronal protein synthesis in hippocampal neurons, and had a significant effect on increasing neuron survival and prolonging survival time in hippocampus Significant effect It is suggested that interleukin-like cytokines rhIL-1β, rhIL-2 and rhIL-6 have a nutritive effect on hippocampal neurons.