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目的研究肿瘤坏死因子(TNF)-α启动子基因多态性与胸腺瘤发病的相关性。方法采用聚合酶链反应加基因测序技术对126例胸腺瘤患者与245名健康对照者TNF-α启动子区进行基因分型,比较TNF-α-863、-308、-238、-806以及-857 5个位点等位基因出现频率的差异。结果胸腺瘤患者TNF-α基因-857位点T等位基因和CT+TT基因型显著高于健康对照组(分别为χ2=6.449,P=0.011;χ2=4.874,P=0.027),而TNF-α-863、-308、-238、-806等位点的等位基因频率与健康对照组比较差异无统计学意义。结论 TNF-α基因-857位点T等位基因与胸腺瘤相关,且其可能为胸腺瘤患者一个新的易感基因标记。
Objective To study the relationship between the polymorphism of tumor necrosis factor (TNF) -α promoter and the incidence of thymoma. Methods The genotypes of TNF-α promoter region in 126 patients with thymoma and 245 healthy controls were genotyped by polymerase chain reaction and gene sequencing. TNF-α-863, -308, -238, -806 and - 857 5 loci allele frequency differences. Results The T allele at -857 site and CT + TT genotype of TNF-α gene in thymoma patients were significantly higher than those in healthy controls (χ2 = 6.449, P = 0.011; χ2 = 4.874, P = 0.027, respectively) The frequencies of alleles of -α-863, -308, -238 and -806 were not significantly different from those of healthy controls. Conclusion The T allele at position -857 of TNF-α gene is associated with thymoma, and it may be a new susceptible gene marker in patients with thymoma.