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目的 观察外源性碱性成纤维细胞生长因子(bFGF)对急性水肿性胰腺炎大鼠的治疗作用,并探讨其作用机制。方法 予雄性SD大鼠间隔1h皮下注射蛙皮素5 .5 μg/kg体重和7.5μg/kg体重诱导急性水肿性胰腺炎,3h后腹腔内注射bFGF 10 0 μg/kg体重。观察各组大鼠胰腺炎生化及病理学等指标的变化。结果 bFGF治疗组大鼠的血清淀粉酶,脂肪酶以及胰腺组织湿/干质量比率(13 83 .0±94.6)、(194.0±43 .6)和(4 .3 2±0 .3 2 )U /L明显低于非治疗组(P <0 .0 1) ,而与空白对照组差异无统计学意义(P >0 .0 5 )。光镜下显示其胰腺组织炎症表现显著改善。免疫组织化学结果显示该组胰腺腺泡细胞被5 溴 2 去氧胞苷标记的细胞核数[(18.9±1.4)个/显微镜视野,n =10 ]明显多于非治疗组(P <0 .0 1)。结论 早期外源性bFGF对蛙皮素诱导的大鼠急性水肿性胰腺炎有显著的治疗作用,其作用机制是通过减轻胰腺炎症和促进胰腺组织再生来实现的
Objective To observe the therapeutic effect of exogenous basic fibroblast growth factor (bFGF) on acute edematous pancreatitis in rats and to explore its mechanism. Methods Male Sprague-Dawley rats were injected subcutaneously with 5 and 5 μg / kg body weight of blebbistatin and 7.5 μg / kg body weight at 1 hour intervals to induce acute edematous pancreatitis. After 3 h, bFGF (100 μg / kg body weight) was intraperitoneally injected. The changes of biochemical and pathological indexes of pancreatitis in rats were observed. Results The wet / dry weight ratio of serum amylase, lipase and pancreas in bFGF treatment group (13 83.0 ± 94.6), (194.0 ± 43.6) and (4.32 ± 0.32) U / L was significantly lower than the non-treatment group (P <0.01), but no significant difference with the control group (P> 0.05). Light microscopy showed significant improvement in pancreatic inflammation. Immunohistochemistry results showed that the number of nuclei labeled with 5-bromodeoxy-2-deoxycytidine in pancreatic acinar cells in this group was significantly higher than that in non-treated group (18.9 ± 1.4) / microscopic field (n = 10) 1). Conclusion Early exogenous bFGF has a significant therapeutic effect on the acute edematous pancreatitis induced by bombesin in rats, and its mechanism of action is through relieving pancreatic inflammation and promoting pancreatic tissue regeneration.