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目的 揭示中枢神经髓鞘脱脂过程在多发性硬化 (MS)发生发展中的作用。方法 以人脱脂神经髓鞘二次致敏诱导MS患者T淋巴细胞系 (MS TCL) ,用髓鞘碱性蛋白 (MBP)、蛋白脂蛋白 (PLP)及其合成多肽等抗原检测TCL的增殖反应。结果 MS组对 11种抗原均有较强反应 ,对 7种抗原的反应性与对照组间有差异 (P <0 0 5 ) ,两组总和平均阳性孔比较P <0 0 0 1(5 49± 5 31与3 10± 3 17比较 )。结论 PLP和MBP与MS发病有关 ;M87 10 6、P95 117、P40 6 0、P185 2 0 6等序列可能是致病抗原决定簇 ;MS病人体内可能有神经髓鞘异常脱脂。
Objective To reveal the role of central nervous myelin degreasing in the development of multiple sclerosis (MS). Methods T lymphocyte cell line (MS TCL) of MS patients was induced by secondary degranulation of myelin sheath. The proliferative response of TCL was detected by antigens such as myelin basic protein (MBP), protein lipoprotein (PLP) and its synthetic peptide . Results The MS group had a strong response to all 11 antigens and the reactivity to 7 antigens was significantly different from that of the control group (P <0 05). The mean total positive wells in the two groups were significantly lower than those in the control group (P <0.001) ± 5 31 vs. 3 10 ± 3 17). Conclusion PLP and MBP are related to the pathogenesis of MS. M87 10 6, P95 117, P40 6 0, P185 2 0 6 sequences may be pathogenic epitopes. MS patients may have abnormal demyelination of myelin sheath.